Rapid and simple detection ofClostridium botulinumtypes A and B by loop-mediated isothermal amplification
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چکیده
منابع مشابه
Rapid detection of infectious bursal disease by loop-mediated isothermal amplification for field analysis
Infectious bursal disease (IBD) is an immunosuppressive, acute and highly contagious illness of growing-poultry stock infected with infectious bursal disease virus (IBDV). It is common in Pakistan, causing potential economic losses throughout the year. The objective of the study is to propose a rapid, sensitive and specific diagnostic tool, and compare it with existing commonly used reverse tra...
متن کاملRapid and simple detection of methicillin-resistance staphylococcus aureus by orfX loop-mediated isothermal amplification assay
BACKGROUND Methicillin-resistant Staphylococcus aureus (MRSA) has become one of the most prevalent pathogens responsible for nosocomial infections throughout the world. As clinical MRSA diagnosis is concerned, current diagnostic methodologies are restricted by significant drawbacks and novel methods are required for MRSA detection. This study aimed at developing a simple loop-mediated isotherma...
متن کاملrapid detection of streptococcus agalactiae infection using a loop-mediated isothermal amplification method
methods genomic dna of cultured s. agalactiae was prepared and loop-mediated isothermal amplification (lamp) primers were designed based on the camp gene in bacteria. the optimum primer set was selected based on the reaction speed and specificity. the reaction result was monitored visually. the sensitivity and specificity of the lamp method were evaluated and compared with polymerase chain reac...
متن کاملSensitive and rapid detection of edwardsiellosis in fish by a loop-mediated isothermal amplification method.
Here we report a rapid and sensitive method (using loop-mediated isothermal amplification [LAMP]) for the diagnosis of edwardsiellosis, a fish disease caused by Edwardsiella tarda, in Japanese flounder. A set of four primers was designed, and conditions for the detection were optimized for the detection of E. tarda in 45 min at 65 degrees C. No amplification of the target hemolysin gene was det...
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ژورنال
عنوان ژورنال: Journal of Applied Microbiology
سال: 2009
ISSN: 1364-5072,1365-2672
DOI: 10.1111/j.1365-2672.2008.04084.x